Molecular biology techniques, with Nicholas Naeger (45 min.)
Nicholas Naeger describes laboratory techniques that BeeSpace
researchers depend upon to generate new knowledge about insect
behaviors. As you view this presentation, look for answers to the
following questions.- Why would a bee behavior researcher conduct an experiment using qRT-PCR?
- Why would a bee behavior researcher conduct an experiment using “in situ” analysis?
- Why would a bee behavior researcher conduct an experiment using microarray analysis?
(Click to view)
Contents
- Introduction (2:35)
- qRT-PCR first steps (2:41)
- RNA extraction (3:27)
- Spectrophotometry (1:18)
- cDNA analysis (2:32)
- PCR (5:58)
- qRT-PCR measurement (2:28)
- In situ analysis (4:04)
- Microarrays (3:22)
- Biology, and more (0:51)
- Questions (15:20)
Direct-download links
- Video in Windows Media (wmv) format (262 mb; requires Windows Media Player)
- Video in Adobe Flash format (78 mb; requires Adobe Flash Player)
- Audio in MP3 format (10 mb; requires MP3 player)
- Transcript in Adobe Acrobat format (132 kb; requires Adobe Acrobat reader)
- Slide set in Adobe Acrobat format (1.0 mb; requires Adobe Acrobat reader)
